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| Enquiries: please contact 01372 378822 or use our enquiry form
Trial period option
The Nuance is currently being offered on a 30 day sale or return basis to allow assessment of the technology in relation to a specific application prior to purchasing.
On-site installation and training will be provided free of charge.
The Nuance can be returned after 30 days if you are not completely satisfied.
 | A full purchase order must be submitted in advance, though will not be invoiced until acceptance after the trial
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| For some fluorescence applications the filter cubes' emission filters will need to be exchanged for a long pass filter (some are available for loan)
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| A 1x C-mount microscope adapter will be required (some are available for loan)
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| Delivery costs will be chargeable
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| This offer is subject to availability and can be withdrawn at any time |
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Nuance™ |
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Multi-label imaging without cross-talk
As biological imaging becomes more about studying complex systems rather than single events, Nuance multispectral imaging systems enable users to quantitate molecular markers even when they are co-localized in a single tissue section, producing clear and accurate images of each individual label on a multi-label tissue section. These systems also offer the powerful capability to unmix and remove autofluorescence in Nuance fluorescence images, thereby dramatically increasing signal-to-noise and improving the accuracy of your results. Nuance systems deliver flow-cytometry-like data while preserving the morphological context down to the sub-cellular level. | |
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Brightfield immunohistochemistry with color re-mapping for legibility. Oral squamous carcinoma (FFPE, 20X) stained for CD34 (endothelium, Nova Red) and podoplanin (VIP, purple), and counterstained with hematoxylin. The spectral dataset was unmixed, the component planes re-colored (purple to green) and displayed as a composite image with improved contrast and legibility over the original.
Sample courtesy Michael Feldman, University of Pennsylvania. |
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There are three Nuance models:
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Nuance EX |
Nuance FX |
Nuance VX |
| Bandwidth Flexibility |
20 and 40 nm |
20 and 40 nm |
20 mm |
| Scientific-Grade CCD |
Cooled Sony ICX285 |
Cooled Sony ICX285 |
Cooled Sony ICX285 |
| Mount Required* |
1x C-mount |
1x C-mount |
1x C-mount |
| Acquisition and Analysis Software |
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| Applications |
| Brightfield |
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| Fluorescence |
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| NIR Fluorescence |
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| *An F-mount macro lens is available for macroscopic applications. |
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| PDF Download |
| Nuance 2 Brochure (PDF, 2,4 MB) |
| Nuance 2 Data Sheet (PDF, 1,5 MB) |
| Nuance 2.4 Imaging Software (PDF, 3,7 MB) |
| Technical Note: Nuance System CCD Specifications (PDF, 2,8 MB) |
| Application Note: Detecting Phenotypical Subtypes of Breast Cancer with Multiplexed Immunohistochemistry (PDF, 1,7 MB) |
| Application Note: In situ miRNA Expression Profiling Using Multispectral Imaging to Elucidate Pathways Involved in Neurodegenerative Disease (PDF, 307 KB) |
| Application Note: Multispectral Imaging and Pathology (PDF, 3,7 MB) |
| Nuance: Selected Fluorescence and Brightfield References (PDF, 109 KB) |
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