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| Measurement chambers |
| Axial flow chamber (QAFC 302) |
Constant flow rate between 0 and 0.2 ml/min without external temperature stabilisation.
Constant flow rate maximum 2 ml/min with external temperature stabilisation. |
| Measurement volume |
~ 80 µl |
| Exchange volume |
optimum ~ 2 ml minimum ~ 0.8 ml |
| Working temperature |
18 to 40°C, stability ± 0.02 K |
| Window chamber (QWIC 301) |
| Measurement volume |
~ 0.40 ml |
| Working distance to microscope |
4 - 8 mm |
| Minimum bench-to-lens distance |
28 mm |
| Working temperature |
18 to 40°C, stability ± 0.03 K |
| Frequency and Dissipation Characteristics |
| Frequency range |
1 - 42 MHz |
| Frequency resolution |
0.01 Hz |
| Mass resolution, using Sauerbrey |
1.8 pg/mm2 (0.18 ng/cm2) |
| Dissipation resolution |
1 x 10-9 |
| Active sensor area |
7mm2 |
| Conditions: |
1 datapoint / 5 seconds, 20-37 °C, water |
| Smallest detectable change in f* |
0.05 Hz corresp. to 0.9 ng/cm2 (9 pg/mm2) |
| Smallest detectable change in D* |
0.04 x 10-6 |
| Conditions: |
4 datapoints / second, 20-37 °C, water |
| Smallest detectable change in f* |
0.2 Hz corresp. to 3.5 ng/cm2 (35 pg/mm2) |
| Smallest detectable change in D* |
0.1 x 10-6 |
| * As comparison, binding of Myoglobin, a small globular protein (Mw 17.8 kD) that forms a rigid monolayer, gives a frequency response of 10 Hz and a Dissipation of ~0.2x10-6. Mefp-1, an elongated protein (Mw 120 kD) forming a soft structure, has a corresponding dissipation of ~12x10-6 and a frequency response of 60 Hz. |
| Max load |
~ 0.1 mg/cm2 |
| Max film thickness |
~ 10 µm (depending on the viscoelastic properties of the material) |
| Software |
| QSoft 301 |
| Operating system |
Windows 98/ME/NT/2000/XP |
| Interface |
Parallel port o |
| Normal rate, low noise |
~ 4 data points per second |
| Maximum output rate |
~ 15 data points per second |
| QTools |
| Viscoelastic models incorporated |
Voight/Maxwell |
| Picture formats |
Bitmap, Metafile, Enhanced Metafile, JPEG |
| Input data |
Frequency and dissipation from min. 2 frequencies |
| Output data |
Modeled values of density, shear modulus, viscosity and thickness |