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Nuance – A Multispectral Imaging System
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Multispectral Imaging is a technique that provides images of a scene at multiple wavelengths and can generate precise optical spectra at every pixel. Image processing software can then be used to extract the maximum information from the spectral image. Multispectral Imaging in the visible and near-infrared wavelength range is routinely used in "remote sensing", the analysis of landscapes from aircrafts, industrial process controll, vegetable sorting and much more.
Multispectral Imaging has also been adapted for use in microscopy, with applications in surgical pathology, multicolor fluorescence immunohistochemistry, and in DNA expression arrays among others. |  |


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| Literature |
| Separation of multiple stains which co-localize in a specimen (PDF, 497 KB) |
| Spectral Imaging for Separation of Fluorescent Signals from Autofluorescence (PDF, 1,2 MB) |
| Visualization of Microscopy-Based Spectral Imaging Data from Multi-Label Tissue Sections (PDF, 953 KB) |
| In Situ miRNA Expression Profiling Using Multi Spectral Imaging to Elucidate Pathways Involved in Neurodegenerative Disease (PDF, 652 KB) |
| Multispectral Imaging in Biology and Medicine: Slices of Life (PDF, 3,3 MB) |
| Multispectral imaging and pathology: seeing and doing more (PDF, 3,2 MB) |
| Multiplexing with Multispectral Imaging: From Mice to Microscopy (PDF, 1,6 MB) |
| Autofluorescence removal, multiplexing and automated analysis methods for in-vivo fluorescence imaging (PDF, 1,7 MB) |
| In situ miRNA Expression Profiling Using Multispectral Imaging to Elucidate Pathways Involved in Neurodegenerative Disease (PDF, 307 KB) |


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